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KMID : 0613820140240090967
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Journal of Life Science
2014 Volume.24 No. 9 p.967 ~ p.972
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Anti-proliferative Activities of Solvent Fractions of Lees Extracts in Human Colorectal HCT116 Cells
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Kang Hyung-Taek
Lee Seung-Hoon
Kim Soon-Young
Kim Mi-Sun
Shin Woo-Chang
Sohn Ho-Yong
Kim Jong-Sik
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Abstract
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In the present study, we prepared eighty-five different kinds of lees extracts and their solvent fractions and investigated their anti-proliferative activities against human colorectal cancer HCT116 cells. HCT116 cells were treated with eighty-five solvent fractions of lees extracts and then cell viability was measured using MTS assay. Among the treated solvent fractions, three solvent fractions (KSD-E1-3, KSD-E2-3, and KSD-E4-3) were selected based on cell viability assay. In addition, we performed an oligo DNA microarray analysis to analyze the gene expression changes by treatment of KSD-E1-3 in HCT116 cells. Among the up-regulated genes, we selected 4 genes (NAG-1, ATF3, p21, and DDIT3) and performed RT-PCR using gene-specific primers. Among the treated solvent fractions, KSD-E1-3 dramatically induced the expressions of the four selected genes. In addition, we investigated whether the up-regulations of those genes were dependent on the transcription factor p53’s presence using p53 null HCT116 cells. The results indicate that the up-regulations of NAG-1, ATF3, and DDIT3 are not dependent on the p53 presence, whereas p21 is dependent on the p53 presence. These findings may help to understand the molecular mechanisms of the anti-proliferative activity mediated by rice wine lees in human colorectal cancer cells.
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KEYWORD
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Anti-proliferation, HCT116 cells, lees, oligo DNA microarray, p53
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